Difference between revisions of "Count reads in transcripts (analysis)"

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==== Description ====
 
==== Description ====
For each transcript count the number mapped NGS reads from BAM file
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For each transcript count the number mapped NGS reads from BAM file.
  
 
==== Parameters: ====
 
==== Parameters: ====
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[[Category:Analyses]]
 
[[Category:Analyses]]
[[Category:BSA (analyses group)]]
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[[Category:Ribosome profiling (analyses group)]]
 
[[Category:ISB analyses]]
 
[[Category:ISB analyses]]
 
[[Category:Autogenerated pages]]
 
[[Category:Autogenerated pages]]

Latest revision as of 18:15, 9 December 2020

Analysis title
Default-analysis-icon.png Count reads in transcripts
Provider
Institute of Systems Biology
Class
CountReadsInTranscripts
Plugin
biouml.plugins.riboseq (RiboSeq Experiment)

[edit] Description

For each transcript count the number mapped NGS reads from BAM file.

[edit] Parameters:

  • BAM track – BAM track with read alignments
  • Transcript set – Transcript set
    • Annotation source – Source of gene annotation
    • Transcripts annotation track – Track with transcripts annotation in BED format
    • Genome sequence – Collection of chromosomal sequences
    • Ensembl – Ensembl database version
    • Transcript subset – Subset of transcripts used in this analysis
    • GTF file – GTF file
  • Strand specific – Count only reads on the same strand as transcript
  • Only overlapping cds – Count only reads that overlap coding sequence
  • Min overlap – Minimal overlap between read and CDS in nucleotides
  • Output table – Output table with read counts for each transcript
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