Find master regulators in networks (GeneWays) (workflow)

Workflow title

Find master regulators in networks (GeneWays)

Provider

geneXplain GmbH

Workflow overview

Description

This workflow is designed to find master regulatory molecules upstream of an input list of genes. Input file is any gene or protein table.

 At the first step, the input table is converted into a table with Entrez Gene IDs.

At the next step the Entrez genes are annotated with additional information, gene description and gene symbols.

The annotated gene table is subjected to master regulator search in the GeneWays network. For each potential master regulator, FDR, Score, and Z-score are calculated.

The results are filtered by Z_Score>1 and Score>0.2 to select statistically significant master regulators.

Further, the filtered regulatory molecules are converted to both Ensembl Gene IDs and a list of UniProt protein IDs.

The table with Ensembl Gene Ids is annotated with additional information, gene description and gene symbols.

Finally, the table with master regulatory molecules is sorted by Score and networks for the three top master regulators are visualized as diagrams in the hierarchical layout.

Parameters

Input gene set

Species

Results folder